Textbook in PDF format

This detailed volume presents a variety of mouse models for cancer studies, from leukemia and lymphoma models to different types of subcutaneous and orthotopic models. Models for the healing process and the assessment of the immune response after local ablative therapies are also included, as well as imaging techniques that allow for the visualization of cancer at the cellular and tissue level. The book closes with a detailed description of necropsy, which is essential for obtaining good biomaterial for translational research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Models of Cancer: Methods and Protocols serves as an ideal guide to these controlled and reproducible model experimental systems to study different aspects of cancer biology, including prevention, development, progression, and treatment.
In Vivo Modeling of Leukemia by Murine Stem Cell Transplantation
NOTCH1-Induced T-Cell Acute Lymphoblastic Leukemia In Vivo Models
Urinary Bladder Cancer Induced by N-Butyl-N-(4-Hydroxybutyl)-Nitrosamine
Establishment of Mouse Orthotopic Urinary Bladder Tumor Model and Its Analysis by Light and Electron Microscopy
Azoxymethane/​Dextran Sodium Sulfate (AOM/​DSS) Model of Colorectal Cancer
Isolating Primary Tumor Cells from the MMTV-PyMT Mouse Model and Their Use in Developing an Orthotopic Mouse Model of Breast Cancer
Transplantable Subcutaneous Tumor Models
Preclinical Mouse Metastatic Model Established Through Induced Lung Metastases
In Vivo Wound Healing Model for Characterization​ of Gene Electrotransfer Effects in Mouse Skin
Partial-Volume Irradiation of Murine Tumors
In Vivo Bioluminescence and Fluorescence Imaging:​ Optical Tool for Cancer Research
Cancer Imaging by Intravital Microscopy:​ The Dorsal Window Chamber Model
Imaging of Extravasation of Splenocytes in the Dorsal Skinfold Window Chamber
Mouse Melanoma Model in Tumor Vaccines and Immunotherapy Research
Immunospot Assessment of T-Cell Responses in Preclinical Tumor Models with Undefined Target Antigens
Necropsy in Mouse Research